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monoclonal antibody mab w6 32  (ATCC)


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    Structured Review

    ATCC monoclonal antibody mab w6 32
    Monoclonal Antibody Mab W6 32, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1024 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+antibody+mab+w6+32/W6%2F32/pm41596735-333-16-20
    Average 96 stars, based on 1024 article reviews
    monoclonal antibody mab w6 32 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: Further Evidence for the Immunosuppressive Activity of Transmembrane Envelope Protein p15E of Porcine Endogenous Retrovirus.
    Article Snippet: .. Expression of MHC class I molecules (HLA-ABC) on 293T cells was detected by indirect staining using monoclonal antibody (mAb) W6/32 (American Type Culture Collection, ATCC, Manassas, VA, USA) and phycoerythrin (PE)-conjugated rat anti-mouse kappa light chain (BD Biosciences). .. Directly labeled mAb CD56-APC (B159; BD Biosciences) in combination with CD107a-PE (H4A3; BD Biosciences) were used to study degranulation of human natural killer (NK) cells in response to 293T cells.

    Article Title: The locus-specific enhancer activity of the class I major histocompatibility complex interferon-responsive element is associated with a gamma-interferon (IFN)-inducible factor distinct from STAT1alpha, p48, and IFN regulatory factor-1.
    Article Snippet: Aliquots of 2 3 107 cells were resuspended in complete medium and incubated for 18 h at 37 °C with or without g-IFN (BioSource, Camarillo, CA) and/or bIFN (Sigma) at the indicated concentrations. .. Class I HLA expression was determined by flow cytometry using the monoclonal antibody (mAb) W6/32 (ATCC, Bethesda, MD). ..

    Article Title: Further Evidence for the Immunosuppressive Activity of Transmembrane Envelope Protein p15E of Porcine Endogenous Retrovirus
    Article Snippet: .. Expression of MHC class I molecules (HLA-ABC) on 293T cells was detected by indirect staining using monoclonal antibody (mAb) W6/32 (American Type Culture Collection, ATCC, Manassas, VA, USA) and phycoerythrin (PE)-conjugated rat anti-mouse kappa light chain (BD Biosciences). .. Directly labeled mAb CD56-APC (B159; BD Biosciences) in combination with CD107a-PE (H4A3; BD Biosciences) were used to study degranulation of human natural killer (NK) cells in response to 293T cells.

    Immunopeptidomics:

    Article Title: Further Evidence for the Immunosuppressive Activity of Transmembrane Envelope Protein p15E of Porcine Endogenous Retrovirus.
    Article Snippet: .. Expression of MHC class I molecules (HLA-ABC) on 293T cells was detected by indirect staining using monoclonal antibody (mAb) W6/32 (American Type Culture Collection, ATCC, Manassas, VA, USA) and phycoerythrin (PE)-conjugated rat anti-mouse kappa light chain (BD Biosciences). .. Directly labeled mAb CD56-APC (B159; BD Biosciences) in combination with CD107a-PE (H4A3; BD Biosciences) were used to study degranulation of human natural killer (NK) cells in response to 293T cells.

    Article Title: Further Evidence for the Immunosuppressive Activity of Transmembrane Envelope Protein p15E of Porcine Endogenous Retrovirus
    Article Snippet: .. Expression of MHC class I molecules (HLA-ABC) on 293T cells was detected by indirect staining using monoclonal antibody (mAb) W6/32 (American Type Culture Collection, ATCC, Manassas, VA, USA) and phycoerythrin (PE)-conjugated rat anti-mouse kappa light chain (BD Biosciences). .. Directly labeled mAb CD56-APC (B159; BD Biosciences) in combination with CD107a-PE (H4A3; BD Biosciences) were used to study degranulation of human natural killer (NK) cells in response to 293T cells.

    Staining:

    Article Title: Further Evidence for the Immunosuppressive Activity of Transmembrane Envelope Protein p15E of Porcine Endogenous Retrovirus.
    Article Snippet: .. Expression of MHC class I molecules (HLA-ABC) on 293T cells was detected by indirect staining using monoclonal antibody (mAb) W6/32 (American Type Culture Collection, ATCC, Manassas, VA, USA) and phycoerythrin (PE)-conjugated rat anti-mouse kappa light chain (BD Biosciences). .. Directly labeled mAb CD56-APC (B159; BD Biosciences) in combination with CD107a-PE (H4A3; BD Biosciences) were used to study degranulation of human natural killer (NK) cells in response to 293T cells.

    Article Title: Further Evidence for the Immunosuppressive Activity of Transmembrane Envelope Protein p15E of Porcine Endogenous Retrovirus
    Article Snippet: .. Expression of MHC class I molecules (HLA-ABC) on 293T cells was detected by indirect staining using monoclonal antibody (mAb) W6/32 (American Type Culture Collection, ATCC, Manassas, VA, USA) and phycoerythrin (PE)-conjugated rat anti-mouse kappa light chain (BD Biosciences). .. Directly labeled mAb CD56-APC (B159; BD Biosciences) in combination with CD107a-PE (H4A3; BD Biosciences) were used to study degranulation of human natural killer (NK) cells in response to 293T cells.

    Flow Cytometry:

    Article Title: The locus-specific enhancer activity of the class I major histocompatibility complex interferon-responsive element is associated with a gamma-interferon (IFN)-inducible factor distinct from STAT1alpha, p48, and IFN regulatory factor-1.
    Article Snippet: Aliquots of 2 3 107 cells were resuspended in complete medium and incubated for 18 h at 37 °C with or without g-IFN (BioSource, Camarillo, CA) and/or bIFN (Sigma) at the indicated concentrations. .. Class I HLA expression was determined by flow cytometry using the monoclonal antibody (mAb) W6/32 (ATCC, Bethesda, MD). ..

    Blocking Assay:

    Article Title: Methods and compositions for treating melanoma
    Article Snippet: .. The percentage of cytotoxicity was determined by the following formula: % cytotoxicity = Experimental release - Spontaneous release Maximal release - Spontaneous release × 100 E. Blocking of Lymphocyte Proliferative Response to BRAFV600E Peptide Stimulation Peptide-pulsed monocytes (proliferation assay) or tumor targets (CTL assay) were incubated with anti-HLA class I-specific monoclonal antibody (mAb) W6/32 (IgG2a; 10 μg/ml), anti-HLA class II-specific mAb B33.1 (both obtained from Dr. B. Perussia, Thomas Jefferson University and Dr. G. Trinchieri, The Wistar Institute); or anti-HLA-B57/HLA-A2 mAb MA2.1 (IgG1; 10 μg/ml) (Takahashi M, et al. Clin Cancer Res 1995; 1:1071-7; American Type Culture Collection, Manassas, Va.). ..

    Proliferation Assay:

    Article Title: Methods and compositions for treating melanoma
    Article Snippet: .. The percentage of cytotoxicity was determined by the following formula: % cytotoxicity = Experimental release - Spontaneous release Maximal release - Spontaneous release × 100 E. Blocking of Lymphocyte Proliferative Response to BRAFV600E Peptide Stimulation Peptide-pulsed monocytes (proliferation assay) or tumor targets (CTL assay) were incubated with anti-HLA class I-specific monoclonal antibody (mAb) W6/32 (IgG2a; 10 μg/ml), anti-HLA class II-specific mAb B33.1 (both obtained from Dr. B. Perussia, Thomas Jefferson University and Dr. G. Trinchieri, The Wistar Institute); or anti-HLA-B57/HLA-A2 mAb MA2.1 (IgG1; 10 μg/ml) (Takahashi M, et al. Clin Cancer Res 1995; 1:1071-7; American Type Culture Collection, Manassas, Va.). ..

    CTL Assay:

    Article Title: Methods and compositions for treating melanoma
    Article Snippet: .. The percentage of cytotoxicity was determined by the following formula: % cytotoxicity = Experimental release - Spontaneous release Maximal release - Spontaneous release × 100 E. Blocking of Lymphocyte Proliferative Response to BRAFV600E Peptide Stimulation Peptide-pulsed monocytes (proliferation assay) or tumor targets (CTL assay) were incubated with anti-HLA class I-specific monoclonal antibody (mAb) W6/32 (IgG2a; 10 μg/ml), anti-HLA class II-specific mAb B33.1 (both obtained from Dr. B. Perussia, Thomas Jefferson University and Dr. G. Trinchieri, The Wistar Institute); or anti-HLA-B57/HLA-A2 mAb MA2.1 (IgG1; 10 μg/ml) (Takahashi M, et al. Clin Cancer Res 1995; 1:1071-7; American Type Culture Collection, Manassas, Va.). ..

    Incubation:

    Article Title: Methods and compositions for treating melanoma
    Article Snippet: .. The percentage of cytotoxicity was determined by the following formula: % cytotoxicity = Experimental release - Spontaneous release Maximal release - Spontaneous release × 100 E. Blocking of Lymphocyte Proliferative Response to BRAFV600E Peptide Stimulation Peptide-pulsed monocytes (proliferation assay) or tumor targets (CTL assay) were incubated with anti-HLA class I-specific monoclonal antibody (mAb) W6/32 (IgG2a; 10 μg/ml), anti-HLA class II-specific mAb B33.1 (both obtained from Dr. B. Perussia, Thomas Jefferson University and Dr. G. Trinchieri, The Wistar Institute); or anti-HLA-B57/HLA-A2 mAb MA2.1 (IgG1; 10 μg/ml) (Takahashi M, et al. Clin Cancer Res 1995; 1:1071-7; American Type Culture Collection, Manassas, Va.). ..



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    Application of C7709A2 mAb for HLA-A24 ligandome analysis. (A) Cell surface expression of HLA class I on K562-A24 and K562. Cells were stained with <t>W6/32</t> (red), C7709A2 (green), or PBS (purple). (B) Schematic workflow of pan-HLA class I or HLA-A24 ligandome analysis. (C) Number of peptides eluted and sequenced from K562-A24 and K562. The number of peptides shared between W6/32 and C7709A2 results is indicated in gray. (D) Length distribution of peptides from K562-A24 using W6/32 and C7709A2. (E) Amino acid-sequence logo of 9-mer peptides from K562-A24. Amino acids are colored as follows: aromatic (F, H, W, Y), green; aliphatic (L, I, V), black; hydrophobic (A, C, G, M, P), blue; charged (D, E, K, R), red; and polar uncharged (N, Q, S, T), purple.
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    Image Search Results


    Application of C7709A2 mAb for HLA-A24 ligandome analysis. (A) Cell surface expression of HLA class I on K562-A24 and K562. Cells were stained with W6/32 (red), C7709A2 (green), or PBS (purple). (B) Schematic workflow of pan-HLA class I or HLA-A24 ligandome analysis. (C) Number of peptides eluted and sequenced from K562-A24 and K562. The number of peptides shared between W6/32 and C7709A2 results is indicated in gray. (D) Length distribution of peptides from K562-A24 using W6/32 and C7709A2. (E) Amino acid-sequence logo of 9-mer peptides from K562-A24. Amino acids are colored as follows: aromatic (F, H, W, Y), green; aliphatic (L, I, V), black; hydrophobic (A, C, G, M, P), blue; charged (D, E, K, R), red; and polar uncharged (N, Q, S, T), purple.

    Journal: Oncoimmunology

    Article Title: HLA-A24 ligandome analysis of colon and lung cancer cells identifies a novel cancer-testis antigen and a neoantigen that elicits specific and strong CTL responses

    doi: 10.1080/2162402X.2017.1293214

    Figure Lengend Snippet: Application of C7709A2 mAb for HLA-A24 ligandome analysis. (A) Cell surface expression of HLA class I on K562-A24 and K562. Cells were stained with W6/32 (red), C7709A2 (green), or PBS (purple). (B) Schematic workflow of pan-HLA class I or HLA-A24 ligandome analysis. (C) Number of peptides eluted and sequenced from K562-A24 and K562. The number of peptides shared between W6/32 and C7709A2 results is indicated in gray. (D) Length distribution of peptides from K562-A24 using W6/32 and C7709A2. (E) Amino acid-sequence logo of 9-mer peptides from K562-A24. Amino acids are colored as follows: aromatic (F, H, W, Y), green; aliphatic (L, I, V), black; hydrophobic (A, C, G, M, P), blue; charged (D, E, K, R), red; and polar uncharged (N, Q, S, T), purple.

    Article Snippet: Monoclonal antibodies (mAb) W6/32 (AbD Serotec) and C7709A2 (gift from Dr. P.G.

    Techniques: Expressing, Staining, Sequencing

    HLA-A24 ligandome analysis of colon and lung cancer cells. (A) Cell surface HLA class I expression of indicated cancer lines: four colon cancers (Colo320, SW480, HCT15/β2m, and HCT116) and three lung cancers (LHK2, Sq-1, and 1–87). Cells were stained with W6/32 (red), C7709A2 (green), or PBS (purple). Insets indicate HLA-A genotypes. (B) Schematic workflow of HLA-A24 ligandome analysis of cancer cells. (C) Numbers of non-redundant HLA-A24 ligands detected from colon and lung cancer lines.

    Journal: Oncoimmunology

    Article Title: HLA-A24 ligandome analysis of colon and lung cancer cells identifies a novel cancer-testis antigen and a neoantigen that elicits specific and strong CTL responses

    doi: 10.1080/2162402X.2017.1293214

    Figure Lengend Snippet: HLA-A24 ligandome analysis of colon and lung cancer cells. (A) Cell surface HLA class I expression of indicated cancer lines: four colon cancers (Colo320, SW480, HCT15/β2m, and HCT116) and three lung cancers (LHK2, Sq-1, and 1–87). Cells were stained with W6/32 (red), C7709A2 (green), or PBS (purple). Insets indicate HLA-A genotypes. (B) Schematic workflow of HLA-A24 ligandome analysis of cancer cells. (C) Numbers of non-redundant HLA-A24 ligands detected from colon and lung cancer lines.

    Article Snippet: Monoclonal antibodies (mAb) W6/32 (AbD Serotec) and C7709A2 (gift from Dr. P.G.

    Techniques: Expressing, Staining